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Image Search Results
Journal: PLoS ONE
Article Title: Responses of a Triple Mutant Defective in Three Iron Deficiency-Induced BASIC HELIX-LOOP-HELIX Genes of the Subgroup Ib(2) to Iron Deficiency and Salicylic Acid
doi: 10.1371/journal.pone.0099234
Figure Lengend Snippet: A, At3g07720; B, CYP82C4 ; C, At3g12900; D, MTPA2 ; E, PPC1 ; F, LHY1 ; G, At1g07050; H, PSAF ; The genes in A–E were identified as potential downstream targets of bHLH subgroup Ib(2) factors, while the genes in F–H indicated a more intense response to –Fe in the mutant (compare to ). 3xbhlh and wild type seedlings were grown for 6 d at + and −Fe and exposed for 6 h to 100 µM SA (+SA) or were mock-treated (−SA). Whole seedlings were harvested for analysis. n = 3; the –Fe cDNA samples were derived from the RNAs used in the microarray ; * indicates a significant change (p<0.05) of −Fe versus +Fe; + indicates a significant change (p<0.05) of 3xbhlh versus WT; § indicates a significant change (p<0.05) of +SA versus –SA. Gene expression was studied using reverse transcription-qPCR.
Article Snippet:
Techniques: Mutagenesis, Derivative Assay, Microarray, Gene Expression, Reverse Transcription
Journal: Microbial Cell Factories
Article Title: Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
doi: 10.1186/1475-2859-13-28
Figure Lengend Snippet: Growth, aromatic intermediates production, and substrate consumption during batch fermentation cultures of E. coli PB12.SA22 strain grown in complex broth. (A) Biomass production (●) Glucose consumption (◆). (B) Residual Phenylalanine (☐), Tyrosine (○), Tryptophan (△). (C) SA (▲), DHS (◆), GA (▼) production. In panel A, numbers in parenthesis indicate the sampling time of biomass for total RNA extraction used for microarray analysis (1), (2) and (3) indicates samples collected at 5, 9, and 44 h of cultivation, respectively.
Article Snippet: Samples were adjusted to a final concentration of total RNA = 1 μ g/ μ L. Aliquots of 20 μ L of total
Techniques: Sampling, RNA Extraction, Microarray